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Methanogenic food web in gut contents of the methane-emitting earthworm Eudrilus eugeniae from Brazil

Title data

Schulz, Kristin ; Hunger, Sindy ; Brown, George G. ; Tsai, Siu M. ; Cerri, Carlos C. ; Conrad, Ralf ; Drake, Harold L.:
Methanogenic food web in gut contents of the methane-emitting earthworm Eudrilus eugeniae from Brazil.
In: The ISME Journal. Vol. 9 (2015) Issue 8 . - pp. 1778-1792.
ISSN 1751-7362
DOI: https://doi.org/10.1038/ismej.2014.262

Abstract in another language

The anoxic saccharide-rich conditions of the earthworm gut provide an ideal transient habitat for ingested microbes capable of anaerobiosis. It was recently discovered that the earthworm Eudrilus eugeniae from Brazil can emit methane (CH4) and that ingested methanogens might be associated with this emission. The objective of this study was to resolve trophic interactions of bacteria and methanogens in the methanogenic food web in gut contents of E. eugeniae. RNA-based stable isotope probing of bacterial 16S rRNA as well as mcrA and mrtA (the alpha subunit of methyl-CoM reductase and its isoenzyme, respectively) of methanogens was performed with [13C]-glucose as a model saccharide in gut contents. Concomitant fermentations were augmented by the rapid consumption of glucose, yielding numerous products including molecular hydrogen (H2), carbon dioxide (CO2), formate, acetate, ethanol, lactate, succinate, and propionate. Aeromonadaceae affiliated facultative aerobes and obligate anaerobes affiliated to Lachnospiraceae, Veillonellaceae, and Ruminococcaceae were associated with the diverse fermentations. Methanogenesis was ongoing during incubations, and 13C-labeling of CH4 verified that supplemental [13C]-glucose derived carbon was dissimilated to CH4. Hydrogenotrophic methanogens affiliated with Methanobacteriaceae and Methanoregulaceae were linked to methanogenesis, and acetogens related to Peptostreptoccocaceae were likewise found to be participants in the methanogenic food web. H2 rather than acetate stimulated methanogenesis in methanogenic gut content enrichments, and acetogens appeared to dissimilate supplemental H2 to acetate in methanogenic enrichments. These findings provide insight on the processes and associated taxa potentially linked to methanogenesis and the turnover of organic carbon in the alimentary canal of methane-emitting E. eugeniae.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER126486
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Jul 2015 05:52
Last Modified: 29 Jul 2015 05:52
URI: https://eref.uni-bayreuth.de/id/eprint/17269