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A mussel polyphenol oxidase-like protein shows thiol-mediated antioxidantactivity

Title data

Wang, Jia ; Suhre, Michael H. ; Scheibel, Thomas:
A mussel polyphenol oxidase-like protein shows thiol-mediated antioxidantactivity.
In: European Polymer Journal. Vol. 113 (April 2019) . - pp. 305-3012.
ISSN 0014-3057
DOI: https://doi.org/10.1016/j.eurpolymj.2019.01.069

Abstract in another language

Marine mussels adhere underwater to a variety of substrates using adhesive proteins with post-translationallymodified amino acids, such as 3,4-dihydroxyphenylalanine (DOPA) residues, as a key chemical signature. DOPAcan auto-oxidize easily in seawater reducing the adhesion strength, but contributing to subsequent cohesion(cross-linking) of the underlying proteins. To maintain both reduced and oxidized forms of DOPA with corre-sponding adhesion and cohesion properties, strict redox regulation is necessary for mussel underwater adhesion.In this study, a full-length polyphenol oxidase-like protein (PPOL) fromMytilusgalloprovincialiswas identifiedafter screening of a mussel foot cDNA library using different degenerated PCR primers. The recombinant PPOL(rPPOL) was successfully produced inEscherichiacoli. The rPPOL exhibits thiol-dependent antioxidant activitysuppressing DOPA oxidation. This finding provides insights into how DOPA chemistry could be regulated andpresumably inspires future applications of DOPA-mediated adhesion materials.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Mussel foot; Polyphenol oxidase-like protein; Recombinant production Antioxidant; Free thiols
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Emerging Fields
Profile Fields > Emerging Fields > Food and Health Sciences
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 20 Jan 2020 11:47
Last Modified: 20 Jan 2020 11:47
URI: https://eref.uni-bayreuth.de/id/eprint/54143