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Aigner, Tamara B. ; Scheibel, Thomas:
Self-Rolling Refillable Tubular Enzyme Containers Made of Recombinant Spider Silk and Chitosan.
In: ACS Applied Materials & Interfaces.
Bd. 11
(2019)
Heft 17
.
- S. 15290-15297.
ISSN 1944-8252
DOI: https://doi.org/10.1021/acsami.9b01654
Abstract
Encapsulation of enzymes is often necessary to stabilize them against environmental conditions or to protect them from other harmful enzymes such as proteases. Here, a refillable spatial confinement system was produced using a fully degradable self-rolling biopolymer bilayer. The enzyme containers comprise spider silk and chitosan and enable one-pot reactions in the micro- to milliliter regime by trapping the enzyme inside the semipermeable tube and allow the substrate and/or product either to diffuse freely or to be entrapped. The tubes are stable toward several organic and aqueous solvents. A one-tube system with esterase-2 was used to establish the system. Further, a two-tube system was applied to mimic enzymatic cascades, where the enzymes have to be separated, because they, for example, inhibit each other. The entrapment mode was also tested in the two-tube system, which is beneficial for toxic products or for obtaining high concentrations of the desired product.