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Bacterial Magnetosomes as Novel Platform for the Presentation of Immunostimulatory, Membrane-Bound Ligands in Cellular Biotechnology

Title data

Mickoleit, Frank ; Jérôme, Valérie ; Freitag, Ruth ; Schüler, Dirk:
Bacterial Magnetosomes as Novel Platform for the Presentation of Immunostimulatory, Membrane-Bound Ligands in Cellular Biotechnology.
In: Advanced Biosystems. (2020) .
ISSN 2366-7478
DOI: https://doi.org/10.1002/adbi.201900231

Project information

Project financing: Deutsche Forschungsgemeinschaft
ERC_Starting Grant - Metamech

Abstract in another language

Cell–cell interactions involving specific membrane proteins are critical triggers in cellular development. Ex vivo strategies to mimic these effects currently use soluble proteins or (recombinant) presenter cells, albeit with mixed results. A promising alternative are bacterial magnetosomes, which can be selectively transformed into cell-free membrane–protein presenters by genetic engineering. In this study, the human CD40 Ligand (CD40L), a key ligand for B cell activation, is expressed on the particle surface. Functionality is demonstrated on sensor cells expressing the human CD40 receptor. Binding of CD40L magnetosomes to these cells triggers a signaling cascade leading to the secretion of embryonic alkaline phosphatase. Concomitantly, the CD40–CD40L interaction is strong enough to allow cell recovery by magnetic sorting. Overall, this study demonstrates the potential of magnetosomes as promising cell-free tools for cellular biotechnology, based on the display of membrane-bound target molecules, thereby creating a biomimetic interaction.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: bacterial magnetosomes; CD40; membrane proteins; nanoparticles; receptor-ligand interaction
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Microbiology > Chair Microbiology - Univ.-Prof. Dr. Dirk Schüler
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 600 Technology
600 Technology, medicine, applied sciences > 610 Medicine and health
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 11 Feb 2020 07:35
Last Modified: 11 Feb 2020 07:35
URI: https://eref.uni-bayreuth.de/id/eprint/54316