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Mickoleit, Frank ; Jérôme, Valérie ; Freitag, Ruth ; Schüler, Dirk:
Bacterial Magnetosomes as Novel Platform for the Presentation of Immunostimulatory, Membrane-Bound Ligands in Cellular Biotechnology.
In: Advanced Biosystems.
Bd. 4
(2020)
Heft 3
.
- 1900231.
ISSN 2366-7478
DOI: https://doi.org/10.1002/adbi.201900231
Volltext
Angaben zu Projekten
| Projekttitel: |
Offizieller Projekttitel Projekt-ID Prozess zur kontrollierten Expansion und Differenzierung von primären humanen B-Lymphozyten außerhalb des menschlichen Körpers. Teil 2: Einsatz in der Bioprozesstechnik 411774929 Entschlüsselung des zytoskelettalen Netzwerks zur Mobilität und Positionierung bakterieller magnetischer Organellen: Neue Akteure und Funktionen 67458534 Horizont 2020 Ohne Angabe |
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| Projektfinanzierung: |
Deutsche Forschungsgemeinschaft Europäischer Forschungsrat |
Abstract
Cell–cell interactions involving specific membrane proteins are critical triggers in cellular development. Ex vivo strategies to mimic these effects currently use soluble proteins or (recombinant) presenter cells, albeit with mixed results. A promising alternative are bacterial magnetosomes, which can be selectively transformed into cell-free membrane–protein presenters by genetic engineering. In this study, the human CD40 Ligand (CD40L), a key ligand for B cell activation, is expressed on the particle surface. Functionality is demonstrated on sensor cells expressing the human CD40 receptor. Binding of CD40L magnetosomes to these cells triggers a signaling cascade leading to the secretion of embryonic alkaline phosphatase. Concomitantly, the CD40–CD40L interaction is strong enough to allow cell recovery by magnetic sorting. Overall, this study demonstrates the potential of magnetosomes as promising cell-free tools for cellular biotechnology, based on the display of membrane-bound target molecules, thereby creating a biomimetic interaction.