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Gas Diffusion Electrodes for Use in an Amperometric Enzyme Biosensor

Title data

Hämmerle, Martin ; Achmann, Sabine ; Moos, Ralf:
Gas Diffusion Electrodes for Use in an Amperometric Enzyme Biosensor.
In: Electroanalysis. Vol. 20 (2008) Issue 21 . - pp. 2279-2286.
ISSN 1521-4109
DOI: https://doi.org/10.1002/elan.200804321

Official URL: Volltext

Project information

Project title:
Project's official titleProject's id
No informationHa 4424/1-3

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

The preparation of gas diffusion electrodes and their use in an amperometric enzyme biosensor for the direct detection of a gaseous analyte is described. The gas diffusion electrodes are prepared by covering a PTFE membrane (thickness 250 μm, pore size 2 μm, porosity 35%) with gold, platinum, or a graphite/PTFE mixture. Gold and platinum are deposited by e-beam sputtering, whereas the graphite/PTFE layer is prepared by vacuum filtration of a respective aqueous suspension. These gas diffusion electrodes are exemplarily implemented as working electrodes in an amperometric biosensor for gaseous formaldehyde containing NAD-dependent formaldehyde dehydrogenase from P. putida [EC. 1.2.1.46] as enzyme and 1,2-naphthoquinone-4-sulfonic acid as electrochemical mediator. The resulting sensors are compared with regard to background current, signal noise, linear range, sensitivity, and detection limit. In this respect, sensors with gold or graphite/PTFE covered membranes outclass ones with platinum for this particular analyte and sensor configuration.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos
Faculties
Faculties > Faculty of Engineering Science > Chair Functional Materials
Profile Fields > Advanced Fields > Advanced Materials
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 21 Jan 2015 11:46
Last Modified: 06 Apr 2016 09:22
URI: https://eref.uni-bayreuth.de/id/eprint/5725