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Amperometric Enzyme-based Biosensor for Direct Detection of Formaldehyde in the Gas Phase: Dependence on Electrolyte Composition

Title data

Achmann, Sabine ; Hämmerle, Martin ; Moos, Ralf:
Amperometric Enzyme-based Biosensor for Direct Detection of Formaldehyde in the Gas Phase: Dependence on Electrolyte Composition.
In: Electroanalysis. Vol. 20 (February 2008) Issue 4 . - pp. 410-417.
ISSN 1521-4109
DOI: https://doi.org/10.1002/elan.200704069

Official URL: Volltext

Project information

Project title:
Project's official titleProject's id
No informationHa 4424/1-1

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

An enzymatic sensor detecting the analyte formaldehyde directly from the gas phase is under investigation. In contrast to existing systems, it enables the quantification of the analyte without prior sampling or accumulation and thus can be used as an online system to monitor the formaldehyde concentration in ambient air. The amperometric sensor depends on the enzymatic conversion of the analyte using formaldehyde dehydrogenase from P. putida [EC.1.2.1.46] as the recognition element. It shows a linear response curve up to 15 ppm, with a detection limit of 0.03 pm (S/N=3). In order to optimize the sensor performance the electrolyte composition within the sensor was varied with respect to pH value, buffer concentration and the addition of Ca2+ and Mg2+ ions. To elucidate the influence of the mediator and the enzyme on the sensor performance the stability and activity of the electrochemical mediator and the enzyme alone was examined separately in these different electrolytes.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos
Faculties
Faculties > Faculty of Engineering Science > Chair Functional Materials
Profile Fields > Advanced Fields > Advanced Materials
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 27 Jan 2015 12:53
Last Modified: 06 Apr 2016 09:38
URI: https://eref.uni-bayreuth.de/id/eprint/5987