Nanomaterial Building Blocks Based on Spider Silk - Oligonucleotide Conjugates

Titelangaben

Humenik, Martin ; Scheibel, Thomas:
Nanomaterial Building Blocks Based on Spider Silk - Oligonucleotide Conjugates.
In: ACS Nano. Bd. 8 (2014) Heft 2 . - S. 1342-1349.
ISSN 1936-086X
DOI: https://doi.org/10.1021/nn404916f

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Abstract

Self-assembling protein nanofibrils are promising structures for the
“bottom-up” fabrication of bionanomaterials. Here, the recombinant protein eADF4(C16),
a variant of Araneus diadematus dragline silk ADF4, which self-assembles into nanofibrils, and
short oligonucleotides were modified for site-specific azide-alkyne coupling. Corresponding
oligonuleotide-eADF4(C16) “click” conjugates were hybridized in linear or branched fashion
according to the designed complementarities of the DNA moieties. Self-assembly properties
of higher ordered structures of the spider silk-DNA conjugates were dominated by the silk
component. Assembled β-sheet rich conjugate fibrils were similar in appearance to fibrils of
unmodified eADF4(C16) but enabled the specific attachment of neutravidin-modified gold nanoparticles on their surface directed by complementary
biotin-oligonucleotides, providing the basis for functionalization of such conjugates.