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Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri

Titelangaben

Cornu, Jean-Yves ; Deinlein, Ulrich ; Höreth, Stephan ; Braun, Manuel ; Schmidt, Holger ; Weber, Michael ; Persson, Daniel P. ; Husted, Søren ; Schjørring, Jan Kofod ; Clemens, Stephan:
Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri.
In: New Phytologist. Bd. 206 (2015) Heft 2 . - S. 738-750.
ISSN 1469-8137
DOI: https://doi.org/10.1111/nph.13237

Angaben zu Projekten

Projektfinanzierung: Deutsche Forschungsgemeinschaft

Abstract

Elevated nicotianamine synthesis in roots of Arabidopsis halleri has been established as a zinc (Zn) hyperaccumulation factor. The main objective of this study was to elucidate the mechanism of nicotianamine-dependent root-to-shoot translocation of metals.
Metal tolerance and accumulation in wild-type (WT) and AhNAS2-RNA interference (RNAi) plants were analysed. Xylem exudates were subjected to speciation analysis and metabolite profiling.
Suppression of root nicotianamine synthesis had no effect on Zn and cadmium (Cd) tolerance but rendered plants nickel (Ni)-hypersensitive. It also led to a reduction of Zn root-to-shoot translocation, yet had the opposite effect on Ni mobility, even though both metals form coordination complexes of similar stability with nicotianamine. Xylem Zn concentrations were positively, yet nonstoichiometrically, correlated with nicotianamine concentrations. Two fractions containing Zn coordination complexes were detected in WT xylem. One of them was strongly reduced in AhNAS2-suppressed plants and coeluted with 67Zn-labelled organic acid complexes. Organic acid concentrations were not responsive to nicotianamine concentrations and sufficiently high to account for complexing the coordinated Zn.
We propose a key role for nicotianamine in controlling the efficiency of Zn xylem loading and thereby the formation of Zn coordination complexes with organic acids, which are the main Zn ligands in the xylem but are not rate-limiting for Zn translocation.

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Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Zusätzliche Informationen: BAYCEER126219
Keywords: Arabidopsis halleri; metal hyperaccumulation; metal tolerance; nicotianamine (NA); organic acids; speciation analysis; xylem transport
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Pflanzenphysiologie
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Pflanzenphysiologie > Lehrstuhl Pflanzenphysiologie - Univ.-Prof. Dr. Stephan Clemens
Profilfelder
Profilfelder > Advanced Fields
Profilfelder > Advanced Fields > Ökologie und Umweltwissenschaften
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER
Forschungseinrichtungen
Forschungseinrichtungen > Forschungszentren
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
500 Naturwissenschaften und Mathematik > 580 Pflanzen (Botanik)
Eingestellt am: 26 Mär 2015 07:42
Letzte Änderung: 16 Mai 2022 15:00
URI: https://eref.uni-bayreuth.de/id/eprint/9318