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Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri

Title data

Cornu, Jean-Yves ; Deinlein, Ulrich ; Höreth, Stephan ; Braun, Manuel ; Schmidt, Holger ; Weber, Michael ; Persson, Daniel ; Husted, Søren ; Schjørring, Jan K. ; Clemens, Stephan:
Contrasting effects of nicotianamine synthase knockdown on zinc and nickel tolerance and accumulation in the zinc/cadmium hyperaccumulator Arabidopsis halleri.
In: New Phytologist. Vol. 206 (2015) Issue 2 . - pp. 738-750.
ISSN 1469-8137
DOI: https://doi.org/10.1111/nph.13237

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Elevated nicotianamine synthesis in roots of Arabidopsis halleri has been established as a zinc (Zn) hyperaccumulation factor. The main objective of this study was to elucidate the mechanism of nicotianamine-dependent root-to-shoot translocation of metals.
Metal tolerance and accumulation in wild-type (WT) and AhNAS2-RNA interference (RNAi) plants were analysed. Xylem exudates were subjected to speciation analysis and metabolite profiling.
Suppression of root nicotianamine synthesis had no effect on Zn and cadmium (Cd) tolerance but rendered plants nickel (Ni)-hypersensitive. It also led to a reduction of Zn root-to-shoot translocation, yet had the opposite effect on Ni mobility, even though both metals form coordination complexes of similar stability with nicotianamine. Xylem Zn concentrations were positively, yet nonstoichiometrically, correlated with nicotianamine concentrations. Two fractions containing Zn coordination complexes were detected in WT xylem. One of them was strongly reduced in AhNAS2-suppressed plants and coeluted with 67Zn-labelled organic acid complexes. Organic acid concentrations were not responsive to nicotianamine concentrations and sufficiently high to account for complexing the coordinated Zn.
We propose a key role for nicotianamine in controlling the efficiency of Zn xylem loading and thereby the formation of Zn coordination complexes with organic acids, which are the main Zn ligands in the xylem but are not rate-limiting for Zn translocation.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER126219
Keywords: Arabidopsis halleri; metal hyperaccumulation; metal tolerance; nicotianamine (NA); organic acids; speciation analysis; xylem transport
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Plant Physiology > Chair Plant Physiology - Univ.-Prof. Dr. Stephan Clemens
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Ecology and the Environmental Sciences
Profile Fields > Advanced Fields > Molecular Biosciences
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 570 Life sciences, biology
500 Science > 580 Plants (Botany)
Date Deposited: 26 Mar 2015 07:42
Last Modified: 12 Feb 2019 09:57
URI: https://eref.uni-bayreuth.de/id/eprint/9318