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Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo

Title data

Schofer, Markus D. ; Tünnermann, Lisa ; Kaiser, Hendric ; Roessler, Philip P. ; Theisen, Christina ; Heverhagen, Johannes T. ; Hering, Jacqueline ; Völker, Maximilian ; Agarwal, Seema ; Efe, Turgay ; Fuchs-Winkelmann, Susanne ; Paletta, Jürgen R. J.:
Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo.
In: Journal of Materials Science: Materials in Medicine. Vol. 23 (September 2012) Issue 9 . - pp. 2227-2233.
ISSN 0957-4530
DOI: https://doi.org/10.1007/s10856-012-4697-0

Abstract in another language

The reconstruction of large bone defects after injury or tumor resection often requires the use of bone substitution. Artificial scaffolds based on synthetic biomaterials can overcome disadvantages of autologous bone grafts, like limited availability and donor side morbidity. Among them, scaffolds based on nanofibers offer great advantages. They mimic the extracellular matrix, can be used as a carrier for growth factors and allow the differentiation of human mesenchymal stem cells. Differentiation is triggered by a series of signaling processes, including integrin and bone morphogenetic protein (BMP), which act in a cooperative manner. The aim of this study was to analyze whether these processes can be remodeled in artificial poly-(l)-lactide acid (PLLA) based nanofiber scaffolds in vivo. Electrospun matrices composed of PLLA-collagen type I or BMP-2 incorporated PLLA-collagen type I were implanted in calvarial critical size defects in rats. Cranial CT-scans were taken 4, 8 and 12 weeks after implantation. Specimens obtained after euthanasia were processed for histology and immunostainings on osteocalcin, BMP-2 and Smad5. After implantation the scaffolds were inhomogeneously colonized and cells were only present in wrinkle- or channel-like structures. Ossification was detected only in focal areas of the scaffold. This was independent of whether BMP-2 was incorporated in the scaffold. However, cells that migrated into the scaffold showed an increased ratio of osteocalcin and Smad5 positive cells compared to empty defects. Furthermore, in case of BMP-2 incorporated PLLA-collagen type I scaffolds, 4 weeks after implantation approximately 40 % of the cells stained positive for BMP-2 indicating an autocrine process of the ingrown cells. These findings indicate that a cooperative effect between BMP-2 and collagen type I can be transferred to PLLA nanofibers and furthermore, that this effect is active in vivo. However, this had no effect on bone formation. The reason for this seems to be an unbalanced colonization of the scaffolds with cells, due to insufficient pore size.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: MESENCHYMAL STEM-CELLS; TISSUE ENGINEERING APPLICATIONS; OSTEOGENIC DIFFERENTIATION; OSTEOBLASTIC DIFFERENTIATION; CELLULAR INFILTRATION; ELECTROSPUN; REGENERATION; PROMOTES; GROWTH; FIBER
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Macromolecular Chemistry II > Chair Macromolecular Chemistry II - Univ.-Prof. Dr. Andreas Greiner
Profile Fields > Advanced Fields > Polymer and Colloid Science
Research Institutions > Research Centres > Bayreuth Center for Colloids and Interfaces - BZKG
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 02 Apr 2015 13:55
Last Modified: 29 May 2015 09:03
URI: https://eref.uni-bayreuth.de/id/eprint/9788