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Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo

Titelangaben

Schofer, Markus D. ; Tünnermann, Lisa ; Kaiser, Hendric ; Roessler, Philip P. ; Theisen, Christina ; Heverhagen, Johannes T. ; Hering, Jacqueline ; Völker, Maximilian ; Agarwal, Seema ; Efe, Turgay ; Fuchs-Winkelmann, Susanne ; Paletta, Jürgen R. J.:
Functionalisation of PLLA nanofiber scaffolds using a possible cooperative effect between collagen type I and BMP-2: impact on colonization and bone formation in vivo.
In: Journal of Materials Science: Materials in Medicine. Bd. 23 (September 2012) Heft 9 . - S. 2227-2233.
ISSN 0957-4530
DOI: https://doi.org/10.1007/s10856-012-4697-0

Abstract

The reconstruction of large bone defects after injury or tumor resection often requires the use of bone substitution. Artificial scaffolds based on synthetic biomaterials can overcome disadvantages of autologous bone grafts, like limited availability and donor side morbidity. Among them, scaffolds based on nanofibers offer great advantages. They mimic the extracellular matrix, can be used as a carrier for growth factors and allow the differentiation of human mesenchymal stem cells. Differentiation is triggered by a series of signaling processes, including integrin and bone morphogenetic protein (BMP), which act in a cooperative manner. The aim of this study was to analyze whether these processes can be remodeled in artificial poly-(l)-lactide acid (PLLA) based nanofiber scaffolds in vivo. Electrospun matrices composed of PLLA-collagen type I or BMP-2 incorporated PLLA-collagen type I were implanted in calvarial critical size defects in rats. Cranial CT-scans were taken 4, 8 and 12 weeks after implantation. Specimens obtained after euthanasia were processed for histology and immunostainings on osteocalcin, BMP-2 and Smad5. After implantation the scaffolds were inhomogeneously colonized and cells were only present in wrinkle- or channel-like structures. Ossification was detected only in focal areas of the scaffold. This was independent of whether BMP-2 was incorporated in the scaffold. However, cells that migrated into the scaffold showed an increased ratio of osteocalcin and Smad5 positive cells compared to empty defects. Furthermore, in case of BMP-2 incorporated PLLA-collagen type I scaffolds, 4 weeks after implantation approximately 40 % of the cells stained positive for BMP-2 indicating an autocrine process of the ingrown cells. These findings indicate that a cooperative effect between BMP-2 and collagen type I can be transferred to PLLA nanofibers and furthermore, that this effect is active in vivo. However, this had no effect on bone formation. The reason for this seems to be an unbalanced colonization of the scaffolds with cells, due to insufficient pore size.

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Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: MESENCHYMAL STEM-CELLS; TISSUE ENGINEERING APPLICATIONS; OSTEOGENIC DIFFERENTIATION; OSTEOBLASTIC DIFFERENTIATION; CELLULAR INFILTRATION; ELECTROSPUN; REGENERATION; PROMOTES; GROWTH; FIBER
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Makromolekulare Chemie II
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Makromolekulare Chemie II > Lehrstuhl Makromolekulare Chemie II - Univ.-Prof. Dr. Andreas Greiner
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Kolloide und Grenzflächen - BZKG
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Profilfelder
Profilfelder > Advanced Fields
Forschungseinrichtungen
Forschungseinrichtungen > Forschungszentren
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
Eingestellt am: 02 Apr 2015 13:55
Letzte Änderung: 29 Mai 2015 09:03
URI: https://eref.uni-bayreuth.de/id/eprint/9788