bei Google ScholarTitelangaben
Nikbakhtzadeh, Mahmood Reza ; Dettner, Konrad ; Boland, Wilhelm ; Dötterl, Stefan:
A novel approach to the quantitation of coeluting cantharidin and deuterium labelled cantharidin in Blister Beetles (Coleoptera: Meloidae).
In: Iranian Journal of Arthropod-Borne Diseases.
Bd. 1
(2007)
Heft 2
.
- S. 19-26.
ISSN 2322-2271
Volltext
Abstract
Blister beetles (Coleoptera: Meloidae) are the main natural source of cantharidin, but the compound titre is depended onseveral factors including, age, sex and mating status of the insects. In order to eliminate such uncertainty factors in physiologicaland chemical studies deuterium labelled cantharidin (D2C) with no natural abundance is normally introduced into thebeetles’ body to use it as a model for studying the cantharidin behaviour in vivo. Experiments were achieved on Mylabrisquadripunctata (Col.: Meloidae) from Southern France and the beetles were exposed to an artificial diet containing a definedamount of D2C. On the other hand, because of the high similarity between the two compounds they cannot be wellquantified by gas chromatography. In order to remove the burden, MRM technique was used for the first time which couldsuccessfully create well-defined cantharidin and D2C peaks and hence a precise measurement. MRM technique was examinedusing a GC-MS Varian Saturn which collected MS/MS data of more than one compound in the same time window ofthe chromatogram. It is especially useful when coeluting compounds have different parent ions, i.e. m/z 84 for D2C (coelutingisotopically-labelled compound) and m/z 82 for cantharidin (beetle-originated compound). Using the routine GC-MSruns, measurement accuracy may be significantly reduced because the D2C peak is covered by the cantharidin huge peakwhile MRM could reveal the two coincided peaks of cantharidin and D2C. Therefore MRM is hereby introduced as themethod of choice to separate cantharidin from D2C with high sensitivity and thus provide a precise base of quantitation.