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Stock, Tilmann ; Selzer, Mirjam ; Rother, Michael:
In vivo requirement of selenophosphate for selenoprotein synthesis in archaea.
In: Molecular Microbiology.
Bd. 75
(2010)
Heft 1
.
- S. 149-160.
ISSN 1365-2958
DOI: https://doi.org/10.1111/j.1365-2958.2009.06970.x
Abstract
Biosynthesis of selenocysteine, the 21st proteinogenicamino acid, occurs bound to a dedicated tRNAin all three domains of life, Bacteria, Eukarya andArchaea, but differences exist between the mechanismemployed by bacteria and eukaryotes/archaea.The role of selenophosphate and the enzyme providingit, selenophosphate synthetase, in archaeal selenoproteinsynthesis was addressed by mutationalanalysis. Surprisingly, MMP0904, encoding a homologueof eukaryal selenophosphate synthetase inMethanococcus maripaludis S2, could not be deletedunless selD, encoding selenophosphate synthetaseof Escherichia coli, was present in trans, demonstratingthat the factor is essential for the organism. Incontrast, the homologous gene of M. maripaludis JJcould be readily deleted, obviating the strain’s abilityto synthesize selenoproteins. Complementing withselD restored selenoprotein synthesis, demonstratingthat the deleted gene encodes selenophosphatesynthetase and that selenophosphate is the in vivoselenium donor for selenoprotein synthesis of thisorganism. We also showed that this enzyme is a selenoproteinitself and that M. maripaludis containsanother, HesB-like selenoprotein previously only predictedfrom genome analyses. The data highlight theuse of genetic methods in archaea for a causal analysisof their physiology and, by comparing two closelyrelated strains of the same species, illustrate the evolutionof the selenium-utilizing trait.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER84123 |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Ökologische Mikrobiologie Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik |
| Eingestellt am: | 29 Jul 2015 05:53 |
| Letzte Änderung: | 11 Feb 2021 08:54 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/17367 |