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Genome-derived criteria for assigning environmental narG and nosZ sequences to operational taxonomic units of nitrate reducers

Title data

Palmer, Katharina ; Drake, Harold L. ; Horn, Marcus A.:
Genome-derived criteria for assigning environmental narG and nosZ sequences to operational taxonomic units of nitrate reducers.
In: Applied and Environmental Microbiology. Vol. 75 (2009) Issue 15 . - pp. 5170-5174.
ISSN 1098-5336
DOI: https://doi.org/10.1128/AEM.00254-09

Abstract in another language

Ninety percent of cultured bacterial nitrate reducers with a 16S rRNA gene similarity of >97% had a narGor nosZ similarity of >67% or >80%, respectively, suggesting that 67% and 80% could be used as standardized,conservative threshold similarity values for narG and nosZ, respectively (i.e., any two sequences that are lesssimilar than the threshold similarity value have a very high probability of belonging to different species), forestimating species-level operational taxonomic units. Genus-level tree topologies of narG and nosZ weregenerally similar to those of the corresponding 16S rRNA genes. Although some genomes contained multiplecopies of narG, recent horizontal gene transfer of narG was not apparent.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER72367
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Jul 2015 05:53
Last Modified: 21 Jul 2021 06:24
URI: https://eref.uni-bayreuth.de/id/eprint/17374