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Kemnitz, Dana ; Kolb, Steffen ; Conrad, Ralf:
High abundance of Crenarchaeota in a temperate acidic forest soil.
In: FEMS Microbiology Ecology.
Bd. 60
(2007)
Heft 3
.
- S. 442-448.
ISSN 1574-6941
DOI: https://doi.org/10.1111/j.1574-6941.2007.00310.x
Abstract
The objective of the study was to elucidate the depth distribution and communitycomposition of Archaea in a temperate acidic forest soil. Numbers of Archaea andBacteria were measured in the upper 18 cm of the soil, and soil cores were sampledon two separate occasions using quantitative PCR targeting 16S rRNA genes.Maximum numbers of Archaea were 0.6–3.8108 16S rRNA genes per gram ofdry soil. Numbers of Bacteria were generally higher, but Archaea always accountedfor a high percentage of the total gene numbers (12–38%). The archaealcommunity structure was analysed by the construction of clone libraries and byterminal restriction length polymorphism (T-RFLP) using the same Archaeaspecificprimers. With the reverse primer labelled, T-RFLP analysis led to thedetection of four T-RFs. Three had lengths of 83, 185 and 218 bp and correspondedto uncultured Crenarchaeota. One (447 bp) was assigned to Thermoplasmales.Labelling of the forward primer allowed further separation of the T-RF intoCrenarchaeota Group I.1c and Group I.1b, and indicated that Crenarchaeota of theGroup I.1c were the predominant 16S rRNA genotype ( 85%) in the soil. Theabundance of Archaea and concentration of ammonia and nitrate decreased withsoil depth. Hence it is unclear if the detected Crenarchaeota Group I.1c participated in ammonia oxidation or had another phenotype.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER39944 |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie > Lehrstuhl Ökologische Mikrobiologie Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Biologie Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik |
| Eingestellt am: | 29 Jul 2015 05:53 |
| Letzte Änderung: | 29 Jul 2015 05:53 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/17396 |