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Scheibel, Thomas ; Kowal, Anthony S. ; Bloom, Jesse ; Lindquist, Susan L.:
Bidirectional amyloid fiber growth for a yeast prion determinant.
In: Current Biology.
Bd. 11
(2001)
Heft 5
.
- S. 366-369.
ISSN 1879-0445
DOI: https://doi.org/10.1016/S0960-9822(01)00099-9
Abstract
The polymerization of many amyloids is a two-stage process initiated by the formation of a seeding nucleus or protofibril. Soluble protein then assembles with these nuclei to form amyloid fibers. Whether fiber growth is bidirectional or unidirectional has been determined for two amyloids. In these cases, bidirectional growth was established by time lapse atomic-force microscopy. Here, we investigated the growth of amyloid fibers formed by NM, the prion-determining region of the yeast protein Sup35p. The conformational changes in NM that lead to amyloid formation in vitro serve as a model for the self-perpetuating conformational changes in Sup35p that allow this protein to serve as an epigenetic element of inheritance in vivo. To assess the directionality of fiber growth, we genetically engineered a mutant of NM so that it contained an accessible cysteine residue that was easily labeled after fiber formation. The mutant protein assembled in vitro with kinetics indistinguishable from those of the wild-type protein and propagated the heritable genetic trait [PSI+] with the same fidelity. In reactions nucleated with prelabeled fibers, unlabeled protein assembled at both ends. Thus, NM fiber growth is bidirectional.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien > Lehrstuhl Biomaterialien - Univ.-Prof. Dr. Thomas Scheibel Profilfelder > Advanced Fields > Neue Materialien Profilfelder > Advanced Fields > Molekulare Biowissenschaften Profilfelder > Advanced Fields > Polymer- und Kolloidforschung Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften Profilfelder Profilfelder > Advanced Fields Profilfelder > Emerging Fields |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 01 Okt 2015 11:48 |
| Letzte Änderung: | 13 Feb 2023 12:26 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19789 |