bei Google ScholarTitelangaben
Kostrewa, Dirk ; Kuhn, Claus-D. ; Engel, C. ; Cramer, Patrick:
An alternative RNA polymerase I structure reveals a dimer hinge.
In: Acta Crystallographica Section D.
Bd. 71
(2015)
Heft 9
.
- S. 1850-1855.
ISSN 1399-0047
DOI: https://doi.org/10.1107/S1399004715012651
Abstract
RNA polymerase I (Pol I) is the central, 14-subunit enzyme that synthesizes the ribosomal RNA (rRNA) precursor in eukaryotic cells. The recent crystal structure of Pol I at 2.8 Å resolution revealed two novel elements: the `expander' in the active-centre cleft and the `connector' that mediates Pol I dimerization [Engel et al. (2013), Nature (London), 502, 650-655]. Here, a Pol I structure in an alternative crystal form that was solved by molecular replacement using the original atomic Pol I structure is reported. The resulting alternative structure lacks the expander but still shows an expanded active-centre cleft. The neighbouring Pol I monomers form a homodimer with a relative orientation distinct from that observed previously, establishing the connector as a hinge between Pol I monomers.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Profilfelder > Advanced Fields > Molekulare Biowissenschaften Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Molekulare Biowissenschaften - BZMB Fakultäten Profilfelder Profilfelder > Advanced Fields Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie |
| Eingestellt am: | 10 Jan 2017 10:45 |
| Letzte Änderung: | 05 Apr 2022 12:13 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/35631 |