at Google ScholarTitle data
Kostrewa, Dirk ; Kuhn, Claus-D. ; Engel, C. ; Cramer, Patrick:
An alternative RNA polymerase I structure reveals a dimer hinge.
In: Acta Crystallographica Section D.
Vol. 71
(2015)
Issue 9
.
- pp. 1850-1855.
ISSN 1399-0047
DOI: https://doi.org/10.1107/S1399004715012651
Abstract in another language
RNA polymerase I (Pol I) is the central, 14-subunit enzyme that synthesizes the ribosomal RNA (rRNA) precursor in eukaryotic cells. The recent crystal structure of Pol I at 2.8 Å resolution revealed two novel elements: the `expander' in the active-centre cleft and the `connector' that mediates Pol I dimerization [Engel et al. (2013), Nature (London), 502, 650-655]. Here, a Pol I structure in an alternative crystal form that was solved by molecular replacement using the original atomic Pol I structure is reported. The resulting alternative structure lacks the expander but still shows an expanded active-centre cleft. The neighbouring Pol I monomers form a homodimer with a relative orientation distinct from that observed previously, establishing the connector as a hinge between Pol I monomers.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences Profile Fields > Advanced Fields > Molecular Biosciences Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB Faculties Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Research Centres |
| Result of work at the UBT: | No |
| DDC Subjects: | 500 Science > 570 Life sciences, biology |
| Date Deposited: | 10 Jan 2017 10:45 |
| Last Modified: | 05 Apr 2022 12:13 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/35631 |