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Farías-Rico, José Arcadio ; Höcker, Birte:
Design of chimeric proteins by combination of subdomain-sized fragments.
In: Keating, Amy E.
(Hrsg.):
Methods in protein design. -
Amsterdam [u.a.]
: Academic Press
,
2013
. - S. 389-405
. - (Methods in Enzymology
; 523
)
ISBN 978-0-12-394292-0
DOI: https://doi.org/10.1016/B978-0-12-394292-0.00018-7
Abstract
Hybrid proteins or chimeras are generated by recombination of protein fragments. In the course of evolution, this mechanism has led to major diversification of protein folds and their functionalities. Similarly, protein engineers have taken advantage of this attractive strategy to build new proteins. Methods that use homologous recombination have been developed to (semi) randomly create chimeras from which the best can be selected. We wanted to recombine very divergent or even unrelated fragments, which is not possible with these methods. Consequently, based on the observation that nature evolves new proteins also through illegitimate recombination, we developed a strategy to design chimeras using protein fragments from different folds. For this approach, we employ detailed structure comparisons, and based on structural similarities, we choose the fragments used for recombination. Model building and minimization can be used to assess the design, and further optimization can be performed using established computational design methodologies. Here, we outline a general approach to rational protein chimera design based on our experience, and provide considerations for the selection of the fragments, the evaluation, and possible redesign of the constructs.
Weitere Angaben
| Publikationsform: | Aufsatz in einem Buch |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie > Lehrstuhl Biochemie III - Proteindesign - Univ.-Prof. Dr. Birte Höcker |
| Titel an der UBT entstanden: | Nein |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik 500 Naturwissenschaften und Mathematik > 540 Chemie |
| Eingestellt am: | 24 Mai 2017 06:50 |
| Letzte Änderung: | 24 Apr 2024 11:09 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/37239 |