at Google ScholarTitle data
Metzner, Julia ; Luckert, Katrin ; Lemuth, Karin ; Hämmerle, Martin ; Moos, Ralf:
Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection.
In: Sensors.
Vol. 18
(2018)
Issue 5
.
- 1309.
ISSN 1424-8220
DOI: https://doi.org/10.3390/s18051309
Abstract in another language
The cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos Profile Fields > Advanced Fields > Advanced Materials Research Institutions > Central research institutes > Bayreuth Center for Material Science and Engineering - BayMAT Faculties Faculties > Faculty of Engineering Science > Chair Functional Materials Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Central research institutes |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 09 May 2018 06:42 |
| Last Modified: | 13 Jun 2024 11:43 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/44059 |