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Amphiphilic Graft Copolymers Capable of Mixed-Mode Interaction as Alternative Nonviral Transfection Agents

Titelangaben

Diaz Ariza, Ivonne L. ; Jérôme, Valérie ; Pérez Pérez, León D. ; Freitag, Ruth:
Amphiphilic Graft Copolymers Capable of Mixed-Mode Interaction as Alternative Nonviral Transfection Agents.
In: ACS Applied Bio Materials. Bd. 4 (2021) Heft 2 . - S. 1268-1282.
ISSN 2576-6422
DOI: https://doi.org/10.1021/acsabm.0c01123

Volltext

Link zum Volltext (externe URL): Volltext

Angaben zu Projekten

Projektfinanzierung: National University of Colombia (grant/award number 36061)
Minciencias Colombia (grant/award number 38527)
Upper Franconian Trust (Oberfrankenstiftung, Bayreuth, Germany; grant/award number 03847)

Abstract

Nonviral gene delivery vectors are attractive candidates compared to viral ones due to their lower cytotoxicity and immunogenicity. However, their efficacy still requires improvement. Major challenges are the effective complexation and protection of the DNA cargo and the intracellular dissociation of the polyplexes at the site of action. It is commonly accepted that polymer architecture and chemistry influence polyplex characteristics and have an impact on the transfection mechanism. We developed a library of biocompatible copolymers based on methoxy poly(ethylene glycol) and a hydrophobic block of poly(ε-caprolactone-co-propargyl carbonate) grafted with a predetermined number of poly(2-(dimethylamino)ethyl methacrylate) segments. Such copolymers could efficiently deliver their cargo even in the presence of serum proteins and to various “difficult to transfect” cells, thereby outperforming the current gold standard 25 kDa linear poly(ethylenimine). Statistical correlation analysis shows that an optimization of the transfection in the case of copolymers combining several interactive functions benefits from treatment as a multiparameter problem.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Hydrophobicity; Gene delivery; Genetics; Polymers; Copolymers
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
600 Technik, Medizin, angewandte Wissenschaften
Eingestellt am: 19 Jan 2021 09:00
Letzte Änderung: 16 Dec 2021 14:10
URI: https://eref.uni-bayreuth.de/id/eprint/62038