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Gorbovytska, Vladyslava ; Kim, Seung-Kyoon ; Kuybu, Filiz ; Götze, Michael ; Um, Dahun ; Kang, Keunsoo ; Pittroff, Andreas ; Brennecke, Theresia ; Schneider, Lisa-Marie ; Leitner, Alexander ; Kim, Tae-Kyung ; Kuhn, Claus-D.:
Enhancer RNAs stimulate Pol II pause release by harnessing multivalent interactions to NELF.
In: Nature Communications.
Bd. 13
(2022)
.
- 2429.
ISSN 2041-1723
DOI: https://doi.org/10.1038/s41467-022-29934-w
Angaben zu Projekten
| Projekttitel: |
Offizieller Projekttitel Projekt-ID Verdrängen eRNAs NELF von RNA polymerase und aktivieren damit die Transkription? Ohne Angabe Open Access Publizieren Ohne Angabe |
|---|---|
| Projektfinanzierung: |
Deutsche Forschungsgemeinschaft KU 3514/1-1 und KU 3514/3-1 |
Abstract
Enhancer RNAs (eRNAs) are long non-coding RNAs that originate from enhancers. Although eRNA transcription is a canonical feature of activated enhancers, the molecular features required for eRNA function and the mechanism of how eRNAs impinge on target gene transcription have not been established. Thus, using eRNA-dependent RNA polymerase II (Pol II) pause release as a model, we examined the requirement of sequence, structure and length of eRNAs for their ability to stimulate Pol II pause release by detaching NELF from paused Pol II. We found eRNA not to exert their function through common structural or sequence motifs. Instead, eRNAs that exhibit a length >200 nucleotides and that contain unpaired guanosines make multiple, allosteric contacts with NELF subunits -A and -E to trigger efficient NELF release. By revealing the molecular determinants of eRNA function, our study establishes eRNAs as an important player in Pol II pause release, and it provides new insight into the regulation of metazoan transcription.