Nanomaterial Building Blocks Based on Spider Silk - Oligonucleotide Conjugates

Title data

Humenik, Martin ; Scheibel, Thomas:
Nanomaterial Building Blocks Based on Spider Silk - Oligonucleotide Conjugates.
In: ACS Nano. Vol. 8 (2014) Issue 2 . - pp. 1342-1349.
ISSN 1936-086X
DOI: https://doi.org/10.1021/nn404916f

Official URL:

Abstract in another language

Self-assembling protein nanofibrils are promising structures for the
“bottom-up” fabrication of bionanomaterials. Here, the recombinant protein eADF4(C16),
a variant of Araneus diadematus dragline silk ADF4, which self-assembles into nanofibrils, and
short oligonucleotides were modified for site-specific azide-alkyne coupling. Corresponding
oligonuleotide-eADF4(C16) “click” conjugates were hybridized in linear or branched fashion
according to the designed complementarities of the DNA moieties. Self-assembly properties
of higher ordered structures of the spider silk-DNA conjugates were dominated by the silk
component. Assembled β-sheet rich conjugate fibrils were similar in appearance to fibrils of
unmodified eADF4(C16) but enabled the specific attachment of neutravidin-modified gold nanoparticles on their surface directed by complementary
biotin-oligonucleotides, providing the basis for functionalization of such conjugates.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Keywords:	assembly . click chemistry . conjugates . fibrils . oligonucleotides . spider silk
Institutions of the University:	Faculties Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Research Institutions > Research Centres > Bayreuth Center for Colloids and Interfaces - BZKG Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB Research Institutions > Research Centres > Research Center for Bio-Macromolecules - BIOmac Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT Faculties > Faculty of Engineering Science > Chair Biomaterials Research Institutions Research Institutions > Research Centres Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Emerging Fields > Food and Health Sciences Profile Fields Profile Fields > Advanced Fields Profile Fields > Emerging Fields
Result of work at the UBT:	Yes
DDC Subjects:	600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited:	27 Feb 2015 11:19
Last Modified:	26 Nov 2015 10:51
URI:	https://eref.uni-bayreuth.de/id/eprint/7594