Spider Silk Coatings as a Bioshield to Reduce Periprosthetic Fibrous Capsule Formation

Titelangaben

Zeplin, Philip H. ; Maksimovikj, Nathalie C. ; Jordan, Martin C. ; Nickel, Joachim ; Lang, Gregor ; Leimer, Axel H. ; Römer, Lin ; Scheibel, Thomas:
Spider Silk Coatings as a Bioshield to Reduce Periprosthetic Fibrous Capsule Formation.
In: Advanced Functional Materials. Bd. 24 (2014) Heft 18 . - S. 2658-2666.
ISSN 1616-3028
DOI: https://doi.org/10.1002/adfm.201302813

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Abstract

Medical grade silicones have been employed for decades in medical applications.
The associated long-term complications, such as capsule formation
and contraction have, however, not been fully addressed yet. The aim
of this study is to elucidate if capsule formation and/or contraction can be
mitigated by veiling the surface of the silicone during the critical phase after
implantation. Medical grade silicone implants are homogeneously coated
with a micrometer thin layer of recombinant spider silk proteins. Biocompatibility
analysis in vitro and in vivo focuses on specifi c physiological reactions.
Applying quantitative methods for the determination of marker-specifi c gene
expression and protein concentration, it is detected that the silk coating
inhibits fi broblast proliferation, collagen I synthesis, and differentiation of
monocytes into CD68-positive histiocytes. It signifi cantly reduces capsule
thickness, post-operative infl ammation, synthesis and re-modeling of extracellular
matrix, and expression of contracture-mediating factors. Therefore,
coatings made of recombinant spider silk proteins considerably reduce major
post-operative complications associated with implantation of silicone-based
alloprosthetics, such as capsular fi brosis and contraction, rendering spider
silk coatings a bioshield for such implants.