at Google ScholarTitle data
Hämmerle, Martin ; Hilgert, Karin ; Moos, Ralf:
Simultaneous determination of laccase activity in a cuvette assay : photometric assay versus oxygen dipping probe.
2023
Event: EBS 2023, 4th European Biosensor Symposium
, August 27 - 30, 2023
, Aachen, Germany.
(Conference item: Conference
,
Poster
)
Abstract in another language
In bio fuel cells, a typical reaction at the cathode is oxygen reduction to water. This reaction can be catalysed by the enzyme laccase. Enzyme activity is often determined in a photometric cuvette assay via monitoring the consumption/ formation of a respective reactant/product of the enzymatic reaction. However, when the enzyme is adsorbed on particulate material in a suspension problems arise due to light scattering. In an alternative approach, laccase activity can be determined via monitoring oxygen depletion. Here, oxygen is quantified by its role as fluorescent quencher using a dipping probe or by a Clark-type oxygen electrode. In this case, no free light path is needed in contrast to the photometric assay above. Thus, suspensions could also be used as samples. In this contribution, we compare the two approaches (photometric assay vs. oxygen dipping probe) by measuring the respective laccase activities simultaneously in a cuvette assay.
Further data
| Item Type: | Conference item (Poster) |
|---|---|
| Refereed: | Yes |
| Institutions of the University: | Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos Profile Fields > Advanced Fields > Advanced Materials Research Institutions > Central research institutes > Bayreuth Center for Material Science and Engineering - BayMAT Faculties Faculties > Faculty of Engineering Science > Chair Functional Materials Profile Fields Profile Fields > Advanced Fields Research Institutions Research Institutions > Central research institutes |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 600 Technology, medicine, applied sciences > 620 Engineering |
| Date Deposited: | 06 Sep 2023 05:43 |
| Last Modified: | 07 Sep 2023 08:47 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/86771 |