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Hämmerle, Martin ; Hilgert, Karin ; Moos, Ralf:
Simultaneous determination of laccase activity in a cuvette assay : photometric assay versus oxygen dipping probe.
2023
Veranstaltung: EBS 2023, 4th European Biosensor Symposium
, August 27 - 30, 2023
, Aachen, Germany.
(Veranstaltungsbeitrag: Kongress/Konferenz/Symposium/Tagung
,
Poster
)
Abstract
In bio fuel cells, a typical reaction at the cathode is oxygen reduction to water. This reaction can be catalysed by the enzyme laccase. Enzyme activity is often determined in a photometric cuvette assay via monitoring the consumption/ formation of a respective reactant/product of the enzymatic reaction. However, when the enzyme is adsorbed on particulate material in a suspension problems arise due to light scattering. In an alternative approach, laccase activity can be determined via monitoring oxygen depletion. Here, oxygen is quantified by its role as fluorescent quencher using a dipping probe or by a Clark-type oxygen electrode. In this case, no free light path is needed in contrast to the photometric assay above. Thus, suspensions could also be used as samples. In this contribution, we compare the two approaches (photometric assay vs. oxygen dipping probe) by measuring the respective laccase activities simultaneously in a cuvette assay.
Weitere Angaben
| Publikationsform: | Veranstaltungsbeitrag (Poster) |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Funktionsmaterialien > Lehrstuhl Funktionsmaterialien - Univ.-Prof. Dr.-Ing. Ralf Moos Profilfelder > Advanced Fields > Neue Materialien Forschungseinrichtungen > Zentrale wissenschaftliche Einrichtungen > Bayreuther Materialzentrum - BayMAT Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Funktionsmaterialien Profilfelder Profilfelder > Advanced Fields Forschungseinrichtungen Forschungseinrichtungen > Zentrale wissenschaftliche Einrichtungen |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 06 Sep 2023 05:43 |
| Letzte Änderung: | 07 Sep 2023 08:47 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/86771 |