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Direct detection of formaldehyde in air by a Novel NAD⁺- and glutathione-independent formaldehyde dehydrogenase-Based biosensor

Title data

Achmann, Sabine ; Hermann, Markus ; Hilbrig, Frank ; Jérôme, Valérie ; Hämmerle, Martin ; Freitag, Ruth ; Moos, Ralf:
Direct detection of formaldehyde in air by a Novel NAD⁺- and glutathione-independent formaldehyde dehydrogenase-Based biosensor.
In: Talanta. Vol. 75 (15 May 2008) Issue 3 . - pp. 786-791.
ISSN 0039-9140
DOI: https://doi.org/10.1016/j.talanta.2007.12.015

Official URL: Volltext

Project information

Project title:
Project's official titleProject's id
No informationHa 4424/1-1

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

An amperometric enzyme-based sensor-system for the direct detection of formaldehyde in air is under investigation. The biosensor is based on a native bacterial NAD+- and glutathione-independent formaldehyde dehydrogenase as biorecognition element. The enzyme was isolated from Hyphomicrobium zavarzinii strain ZV 580, grown on methylamine hydrochloride in a fed-batch process. The sensor depends on the enzymatic conversion of the analyte to formic acid. Released electrons are detected in an amperometric measurement at 0.2 V vs. Ag/AgCl reference electrode by means of a redox-mediator. To optimize the sensing device, Ca2+ and pyrroloquinoline quinone (PQQ) were added to the buffer solution as reconstitutional substances.

At this stage, the sensor shows linear response in the tested ppm-range with a sensitivity of 0.39 μA/ppm. The signal is highly reproducible with respect to sensitivity and base line signal. Reproducibility of sensitivity is more than 90% within the same bacterial batch and even when enzyme of different bacterial batches is used.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: Special Section: Remote Sensing
Keywords: Hyphomicrobium zavarzinii
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Functional Materials
Faculties > Faculty of Engineering Science > Chair Functional Materials > Chair Functional Materials - Univ.-Prof. Dr.-Ing. Ralf Moos
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Advanced Materials
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center for Material Science and Engineering - BayMAT
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: Yes
DDC Subjects: 500 Science
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 27 Jan 2015 10:39
Last Modified: 07 Apr 2016 07:40
URI: https://eref.uni-bayreuth.de/id/eprint/5982