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Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells

Title data

Rudolph, Julia ; Völkl, Matthias ; Jérôme, Valérie ; Scheibel, Thomas ; Freitag, Ruth:
Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells.
In: Scientific Reports. Vol. 11 (3 August 2021) Issue 1 .
ISSN 2045-2322
DOI: https://doi.org/10.1038/s41598-021-95073-9

Project information

Project title:
Project's official titleProject's id
SFB 1357 MikroplastikSFB1357

Project financing: Deutsche Forschungsgemeinschaft
Bayreuth Graduate School

Abstract in another language

Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: microplastics; cells; PS; biomarker; murine macrophages; epithelial cells; cytotoxicity; proliferation; cellular stress; ROS; MTT; Flow Cytometer
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 610 Medicine and health
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 10 Aug 2021 11:33
Last Modified: 10 Aug 2021 11:33
URI: https://eref.uni-bayreuth.de/id/eprint/66737