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Crystallization and preliminary X-ray diffractionanalysis of proximal thread matrix protein 1(PTMP1) from Mytilus galloprovincialis

Title data

Suhre, Michael H. ; Scheibel, Thomas ; Steegborn, Clemens ; Gertz, Melanie:
Crystallization and preliminary X-ray diffractionanalysis of proximal thread matrix protein 1(PTMP1) from Mytilus galloprovincialis.
In: Acta Crystallographica Section F. Vol. 70 (June 2014) Issue 6 . - pp. 769-772.
ISSN 2053-230X
DOI: https://doi.org/10.1107/S2053230X14006165

Official URL: Volltext

Abstract in another language

In order to deal with the dynamic ocean environment, blue mussels adhere to various surfaces via their collagenous byssal threads. PTMP1 (proximal thread matrix protein 1) is one identified matrix protein residing in the proximal thread and is capable of collagen binding. Its sequence comprises two von Willebrand factor type A-like repeats. In order to characterize the structure and domain architecture of PTMP1, recombinant protein was crystallized by vapour diffusion. The obtained crystals diffracted to 1.95 Å resolution and belonged to space group P2₁, with unit-cell parameters a=62.0, b=62.3, c=122.6 Å, β=102.2°. The Matthews coefficient suggested the presence of two monomers in the asymmetric unit and 48.3% solvent content.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Mytilus galloprovincialis; byssal thread; matrix protein; mussel byssus; proximal thread matrix protein 1
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Faculties
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 03 Mar 2015 14:48
Last Modified: 26 Nov 2015 10:51
URI: https://eref.uni-bayreuth.de/id/eprint/7756