ERef Bayreuth

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Resonance assignment of an engineered amino-terminal domain of a major ampullate spider silk with neutralized charge cluster

Title data

Schaal, Daniel ; Bauer, Joschka ; Schweimer, Kristian ; Scheibel, Thomas ; Rösch, Paul ; Schwarzinger, Stephan:
Resonance assignment of an engineered amino-terminal domain of a major ampullate spider silk with neutralized charge cluster.
In: Biomolecular NMR Assignments. Vol. 10 (2016) . - pp. 199-202.
ISSN 1874-270X
DOI: https://doi.org/10.1007/s12104-016-9666-y

Abstract in another language

Spider dragline fibers are predominantly made out of the major ampullate spidroins (MaSp) 1 and 2. The assembly of dissolved spidroin into a stable fiber is highly controlled for example by dimerization of its amino-terminal domain (NRN) upon acidification, as well as removal of sodium chloride along the spinning duct. Clustered residues D39, E76 and E81 are the most highly conserved residues of the five-helix bundle, and they are hypothesized to be key residues for switching between a monomeric and a dimeric conformation. Simultaneous replacement of these residues by their non-titratable analogues results in variant D39N/E76Q/E81Q, which is supposed to fold into an intermediate conformation between that of the monomeric and the dimeric state at neutral pH. Here we report the resonance assignment of Latrodectus hesperus NRN variant D39N/E76Q/E81Q at pH 7.2 obtained by high-resolution triple resonance NMR spectroscopy

Further data

Item Type:	Article in a journal
Refereed:	Yes
Keywords:	Spider silk; Amino-terminal domain; Major ampullate spidroin 1; Dimerization; Latrodectus hesperus; Acidic charge cluster
Institutions of the University:	Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Apl. Prof. Dr. Birgitta Wöhrl Faculties > Faculty of Engineering Science Faculties > Faculty of Engineering Science > Chair Biomaterials Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel Profile Fields Profile Fields > Advanced Fields Profile Fields > Advanced Fields > Polymer and Colloid Science Profile Fields > Advanced Fields > Advanced Materials Profile Fields > Advanced Fields > Molecular Biosciences Profile Fields > Emerging Fields Profile Fields > Emerging Fields > Food and Health Sciences Research Institutions Research Institutions > Central research institutes Research Institutions > Central research institutes > Research Center for Bio-Macromolecules - BIOmac Research Institutions > Central research institutes > Bayreuth Center for Material Science and Engineering - BayMAT Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry - Univ.-Prof. Dr. Janosch Hennig
Result of work at the UBT:	Yes
DDC Subjects:	500 Science > 540 Chemistry 500 Science > 570 Life sciences, biology 600 Technology, medicine, applied sciences 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited:	24 Feb 2016 13:23
Last Modified:	22 Dec 2023 12:24
URI:	https://eref.uni-bayreuth.de/id/eprint/31128