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Hämmerle, Martin ; Hilgert, Karin ; Horn, Marcus A. ; Moos, Ralf:
Analysis of volatile alcohols in apple juices by an electrochemical biosensor measuring in the headspace above the liquid.
In: Sensors and Actuators B: Chemical.
Bd. 158
(2011)
Heft 1
.
- S. 313-318.
ISSN 0925-4005
DOI: https://doi.org/10.1016/j.snb.2011.06.026
Volltext
Angaben zu Projekten
| Projekttitel: |
Offizieller Projekttitel Projekt-ID Ohne Angabe HA4424/1-3 |
|---|---|
| Projektfinanzierung: |
Deutsche Forschungsgemeinschaft |
Abstract
An electrochemical biosensor was optimised for the analysis of volatile alcohols directly from the gas phase without prior absorption or pre-concentration. The sensor is based on the alcohol oxidase (Pichia pastoris) catalyzed conversion of ethanol and the amperometric detection of the generated hydrogen peroxide. Key part of the three-electrode set-up was a gas-diffusion working electrode (potential: +600 mV vs. Ag/AgCl) that consisted of a porous Teflon membrane coated with a thin platinum layer. Headspace samples were analysed for alcohols and used to derive alcohol concentrations in the liquid phase. The biosensor had a sensitivity of 3.43 μA/mM for ethanol, a response time of 69 s, a linear dynamic range of 0.10 – 30 mM, a theoretical detection limit (3 < S/N) of 9.9 μM, and a stability of 86% during continuous operation (18 h @ 1 mM ethanol). Using one sensor on three consecutive days, the mean coefficient of variation was 1.3% (three measurements each day @ 10 mM ethanol). Alcohol contents of three apple juices determined with the biosensor were in the range 0.30 g/l – 0.67 g/l (equivalent to 6.51 mM – 14.5 mM). However, ethanol contents determined by high pressure liquid chromatography coupled to refractive index detection (HPLC-RI) and by a commercial enzyme test kit based on alcohol dehydrogenase ranged from 0.12 g/l to 0.38 g/l (equivalent to 2.60 mM – 8.25 mM). Both indicate that the biosensor detected alcohols other than ethanol in the apple juices. HPLC-RI coupled to the biosensor in a flow-through configuration demonstrated that the biosensor detected methanol concomitant to ethanol. Thus, the biosensor could perform a qualitative analysis of the total content of volatile alcohols in apple juices by analysing the gas phase above the sample. This offers the additional advantage that possible, non-volatile interfering substances in the liquid sample cannot impair the measurement.