at Google ScholarTitle data
Rudolph, Julia ; Völkl, Matthias ; Jérôme, Valérie ; Scheibel, Thomas ; Freitag, Ruth:
Noxic effects of polystyrene microparticles on murine macrophages and epithelial cells.
In: Scientific Reports.
Vol. 11
(2021)
.
- 15702.
ISSN 2045-2322
DOI: https://doi.org/10.1038/s41598-021-95073-9
Project information
| Project title: |
Project's official title Project's id SFB 1357 Mikroplastik SFB1357 Open Access Publizieren No information |
|---|---|
| Project financing: |
Deutsche Forschungsgemeinschaft Bayreuth Graduate School |
Abstract in another language
Microplastic (MP) contamination has been identified as an ecological problem with an increasing impact on everyday life. Yet, possible effects of MP at the cellular level are still poorly understood. Here, the interaction of murine macrophages (J774A.1, ImKC) and epithelial cells (STC-1, BNL CL.2) with well-characterized poly(styrene) MP particles (MPP) of varying sizes (0.2–6.0 µm) was studied. Macrophages are expected to actively engulf particles which could be confirmed in this study, while epithelial cells are found in tissues with direct contact with ingested or inhaled MPP. Here, the epithelial cells from both investigated cell lines did not ingest MPP in significant numbers. Concomitantly, no cytotoxic effects nor any influence on cellular proliferation were observed. Cells from the two macrophage cell lines showed high ingestion of MPP of all sizes, but cytotoxic effects were observed only for one of them (ImKC) and only at MPP concentrations above 250 µg/mL. Indications of cellular stress as well as effects on cell proliferation were observed for cell populations with high particle cell interactions.