Veröffentlichungen "Stemmann, Olaf"

2024

Hellmuth, Susanne ; Stemmann, Olaf:
In vitro reconstitution reveals requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin.
bioRxiv , 2024
DOI: https://doi.org/10.1101/2024.03.12.584565

Hellmuth, Susanne ; Stemmann, Olaf:
Requirement of Nek2a and cyclin A2 for Wapl-dependent removal of cohesin from prophase chromatin.
In: The EMBO Journal. Bd. 43 (2024) . - S. 5237-5259.
ISSN 1460-2075
DOI: https://doi.org/10.1038/s44318-024-00228-9

2023

Abdel-Salam, Ghada M. H. ; Hellmuth, Susanne ; Gradhand, Elise ; Käseberg, Stephan ; Winter, Jennifer ; Pabst, Ann-Sophie ; Eid, Maha M. ; Thiele, Holger ; Nürnberg, Peter ; Budde, Birgit S. ; Toliat, Mohammad Reza ; Brecht, Ines B. ; Schroeder, Christopher ; Gschwind, Axel ; Ossowski, Stephan ; Häuser, Friederike ; Rossmann, Heidi ; Abdel-Hamid, Mohamed S. ; Hegazy, Ibrahim ; Mohamed, Ahmed G. ; Schneider, Dominik T. ; Bertoli-Avella, Aida M. ; Bauer, Peter ; Pearring, Jillian N. ; Pfundt, Rolph ; Hoischen, Alexander ; Gilissen, Christian ; Strand, Dennis ; Zechner, Ulrich ; Tashkandi, Soha A. ; Faqeih, Eissa A. ; Stemmann, Olaf ; Strand, Susanne ; Bolz, Hanno J.:
Biallelic MAD2L1BP (p31ᶜᵒᵐᵉᵗ) mutation is associated with mosaic aneuploidy and juvenile granulosa cell tumors.
In: JCI Insight. Bd. 8 (2023) Heft 22 . - e170079.
ISSN 2379-3708
DOI: https://doi.org/10.1172/jci.insight.170079

2020

Hellmuth, Susanne ; Gómez-H., Laura ; Pendás, Alberto M. ; Stemmann, Olaf:
Securin-independent regulation of separase by checkpoint-induced shugoshin–MAD2.
In: Nature. Bd. 580 (2020) . - S. 536-541.
ISSN 1476-4687
DOI: https://doi.org/10.1038/s41586-020-2182-3

Hellmuth, Susanne ; Stemmann, Olaf:
Separase-triggered apoptosis enforces minimal length of mitosis.
In: Nature. Bd. 580 (2020) . - S. 542-547.
ISSN 1476-4687
DOI: https://doi.org/10.1038/s41586-020-2187-y

2019

Henschke, Lars ; Frese, Matthias ; Hellmuth, Susanne ; Marx, Andreas ; Stemmann, Olaf ; Mayer, Thomas U.:
Identification of Bioactive Small Molecule Inhibitors of Separase.
In: ACS Chemical Biology. Bd. 14 (2019) Heft 10 . - S. 2155-2159.
ISSN 1554-8937
DOI: https://doi.org/10.1021/acschembio.9b00661

2018

Wild, Thomas ; Budzowska, Magda ; Hellmuth, Susanne ; Eibes, Susana ; Karemore, Gopal ; Barisic, Marin ; Stemmann, Olaf ; Choudhary, Chunaram:
Deletion of APC7 or APC16 Allows Proliferation of Human Cells without the Spindle Assembly Checkpoint.
In: Cell Reports. Bd. 25 (2018) Heft 9 . - S. 2317-2328.
ISSN 2211-1247
DOI: https://doi.org/10.1016/j.celrep.2018.10.104

Hellmuth, Susanne ; Gutiérrez-Caballero, Cristina ; Llano, Elena ; Pendás, Alberto M. ; Stemmann, Olaf:
Local activation of mammalian separase in interphase promotes double-strand break repair and prevents oncogenic transformation.
In: The EMBO Journal. Bd. 37 (2018) Heft 22 . - e99184.
ISSN 1460-2075
DOI: https://doi.org/10.15252/embj.201899184

Wolf, Peter G. ; Ramos, Alexander Cuba ; Kenzel, Julia ; Neumann, Brigitte ; Stemmann, Olaf:
Studying meiotic cohesin in somatic cells reveals that Rec8-containing cohesin requires Stag3 to function and is regulated by Wapl and sororin.
In: Journal of Cell Science. Bd. 131 (2018) Heft 11 . - jcs.212100.
ISSN 1477-9137
DOI: https://doi.org/10.1242/jcs.212100

2017

Ferencz, Csilla Maria ; Guigas, Gernot ; Veres, Andreas ; Neumann, Brigitte ; Stemmann, Olaf ; Weiss, Matthias:
In Vitro Reconstitution of the Endoplasmic Reticulum.
In: Current Protocols in Cell Biology. Bd. 76 (2017) . - 11.22.1–11.22.16.
ISSN 1934-2500
DOI: https://doi.org/10.1002/cpcb.30

2016

Ferencz, Csilla Maria ; Guigas, Gernot ; Veres, Andreas ; Neumann, Brigitte ; Stemmann, Olaf ; Weiss, Matthias:
Shaping the endoplasmic reticulum in vitro.
In: Biochimica et Biophysica Acta : Biomembranes. Bd. 1858 (2016) Heft 9 . - S. 2035-2040.
ISSN 1879-2642
DOI: https://doi.org/10.1016/j.bbamem.2016.06.004

2015

Mohr, Lisa ; Buheitel, Johannes ; Schöckel, Laura ; Karalus, Dorothea ; Mayer, Bernd ; Stemmann, Olaf:
An Alternatively Spliced Bifunctional Localization Signal Reprograms Human Shugoshin 1 to Protect Centrosomal Instead of Centromeric Cohesin.
In: Cell Reports. Bd. 12 (2015) Heft 12 . - S. 2156-2168.
ISSN 2211-1247
DOI: https://doi.org/10.1016/j.celrep.2015.08.045

Hellmuth, Susanne ; Rata, Scott ; Brown, Andreas ; Heidmann, Stefan ; Novak, Bela ; Stemmann, Olaf:
Human Chromosome Segregation Involves Multi-Layered Regulation of Separase by the Peptidyl-Prolyl-Isomerase Pin1.
In: Molecular Cell. Bd. 58 (2015) Heft 3 . - S. 495-506.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2015.03.025

Hellmuth, Susanne ; Pöhlmann, Christopher ; Brown, Andreas ; Böttger, Franziska ; Sprinzl, Mathias ; Stemmann, Olaf:
Positive and Negative Regulation of Vertebrate Separase by Cdk1-Cyclin B1 May Explain Why Securin Is Dispensable.
In: The Journal of Biological Chemistry. Bd. 290 (2015) Heft 12 . - S. 8002-8010.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M114.615310

2014

Hellmuth, Susanne ; Böttger, Franziska ; Pan, Cuiping ; Mann, Matthias ; Stemmann, Olaf:
PP2A delays APC/C-dependent degradation of separase-associated but not free securin.
In: The EMBO Journal. Bd. 33 (2014) Heft 10 . - S. 1134-1147.
ISSN 1460-2075
DOI: https://doi.org/10.1002/embj.201488098

2013

Rattani, Ahmed ; Wolna, Magda ; Ploquin, Mickael ; Helmhart, Wolfgang ; Morrone, Seamus ; Mayer, Bernd ; Godwin, Jonathan ; Xu, Wenqing ; Stemmann, Olaf ; Pendás, Alberto M. ; Nasmyth, Kim:
Sgol2 provides a regulatory platform that coordinates essential cell cycle processes during meiosis I in oocytes.
In: eLife. Bd. 2 (5 November 2013) . - e01133.
ISSN 2050-084X
DOI: https://doi.org/10.7554/eLife.01133.001

Buheitel, Johannes ; Stemmann, Olaf:
Prophase pathway-dependent removal of cohesin from human chromosomes requires opening of the Smc3-Scc1 gate.
In: The EMBO Journal. Bd. 32 (2013) Heft 5 . - S. 666-676.
ISSN 1460-2075
DOI: https://doi.org/10.1038/emboj.2013.7

2012

Touati, Sandra A. ; Cladière, Damien ; Lister, Lisa M. ; Leontiou, Ioanna ; Chambon, Jean-Philippe ; Rattani, Ahmed ; Böttger, Franziska ; Stemmann, Olaf ; Nasmyth, Kim ; Herbert, Mary ; Wassermann, Katja:
Cyclin A2 Is Required for Sister Chromatid Segregation, But Not Separase Control, in Mouse Oocyte Meiosis.
In: Cell Reports. Bd. 2 (2012) Heft 5 . - S. 1077-1087.
ISSN 2211-1247
DOI: https://doi.org/10.1016/j.celrep.2012.10.002

2011

Franz, André ; Orth, Michael ; Pirson, Paul A. ; Sonneville, Remi ; Blow, J. Julian ; Gartner, Anton ; Stemmann, Olaf ; Hoppe, Thorsten:
CDC-48/p97 Coordinates CDT1 Degradation with GINS Chromatin Dissociation to Ensure Faithful DNA Replication.
In: Molecular Cell. Bd. 44 (7 Oktober 2011) Heft 1 . - S. 85-96.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2011.08.028

Schöckel, Laura ; Möckel, Martin ; Mayer, Bernd ; Boos, Dominik ; Stemmann, Olaf:
Cleavage of cohesin rings coordinates the separation of centrioles and chromatids.
In: Nature Cell Biology. Bd. 13 (10 Juli 2011) . - S. 966-972.
ISSN 1476-4679
DOI: https://doi.org/10.1038/ncb2280

Hormanseder, Eva ; Tischer, Thomas ; Heubes, Simone ; Stemmann, Olaf ; Mayer, Thomas U.:
Non-proteolytic ubiquitylation counteracts the APC/C-inhibitory function of XErp1.
In: EMBO Reports. Bd. 12 (12 Mai 2011) Heft 5 . - S. 436-443.
ISSN 1469-3178
DOI: https://doi.org/10.1038/embor.2011.32

Orth, Michael ; Mayer, Bernd ; Rehm, Kinga ; Rothweiler, Ulli ; Heidmann, Doris ; Holak, Tad A. ; Stemmann, Olaf:
Shugoshin is a Mad1/Cdc20-like interactor of Mad2.
In: The EMBO Journal. Bd. 30 (2011) . - S. 2868-2880.
ISSN 1460-2075
DOI: https://doi.org/10.1038/emboj.2011.187

2010

Wang, Lily Hui-Ching ; Mayer, Bernd ; Stemmann, Olaf ; Nigg, Erich A.:
Centromere DNA decatenation depends on cohesin removal and is required for mammalian cell division.
In: Journal of Cell Science. Bd. 123 (2010) Heft 5 . - S. 806-813.
ISSN 1477-9137
DOI: https://doi.org/10.1242/jcs.058255

2009

Kudo, Nobuaki R. ; Anger, Martin ; Peters, Antoine H. ; Stemmann, Olaf ; Theussl, Hans-Christian ; Helmhart, Wolfgang ; Kudo, Hiromi ; Heyting, Christa ; Nasmyth, Kim:
Role of cleavage by separase of the Rec8 kleisin subunit of cohesin during mammalian meiosis I.
In: Journal of Cell Science. Bd. 122 (2009) Heft 15 . - S. 2686-2698.
ISSN 1477-9137
DOI: https://doi.org/10.1242/jcs.035287

2008

Daub, Henrik ; Olsen, Jesper V. ; Bairlein, Michaela ; Gnad, Florian ; Oppermann, Felix S. ; Korner, Roman ; Greff, Zoltán ; Keri, György ; Stemmann, Olaf ; Mann, Matthias:
Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.
In: Molecular Cell. Bd. 31 (8 August 2008) Heft 3 . - S. 438-448.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2008.07.007

Rothweiler, Ulli ; Czarna, Anna ; Weber, Lutz ; Popowicz, Grzegorz M. ; Brongel, Kinga ; Kowalska, Kaja ; Orth, Michael ; Stemmann, Olaf ; Holak, Tad A.:
NMR screening for lead compounds using tryptophan-mutated proteins.
In: Journal of Medicinal Chemistry. Bd. 51 (2008) Heft 16 . - S. 5035-5042.
ISSN 1520-4804
DOI: https://doi.org/10.1021/jm8002813

Boos, Dominik ; Kuffer, Christian ; Lenobel, Rene ; Korner, Roman ; Stemmann, Olaf:
Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase.
In: The Journal of Biological Chemistry. Bd. 283 (2008) Heft 2 . - S. 816-823.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M706748200

Nabti, Ibtissem ; Reis, Alexandra ; Levasseur, Mark ; Stemmann, Olaf ; Jones, Keith T.:
Securin and not CDK1/cyclin B1 regulates sister chromatid disjunction during meiosis II in mouse eggs.
In: Developmental Biology. Bd. 321 (2008) Heft 2 . - S. 379-386.
ISSN 1095-564X
DOI: https://doi.org/10.1016/j.ydbio.2008.06.036

2007

Heubes, Simone ; Stemmann, Olaf:
The AAA-ATPase p97Ufd1/Npl4 Is Required for ERAD but Not for Spindle Disassembly in Xenopus Egg Extracts.
In: Journal of Cell Science. Bd. 120 (2007) Heft 8 . - S. 1325-1329.
ISSN 1477-9137
DOI: https://doi.org/10.1242/jcs.006924

Holland, Andrew J. ; Böttger, Franziska ; Stemmann, Olaf ; Taylor, Stephen S.:
Protein phosphatase 2A and separase form a complex regulated by separase autocleavage.
In: The Journal of Biological Chemistry. Bd. 282 (2007) Heft 34 . - S. 24623-24632.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M702545200

2006

Gorr, Ingo H. ; Reis, Alexandra ; Boos, Dominik ; Wühr, Martin ; Madgwick, Suzanne ; Jones, Keith T. ; Stemmann, Olaf:
Essential Cdk1-inhibitory role for separase during meiosis I in vertebrate oocytes.
In: Nature Cell Biology. Bd. 8 (8 September 2006) . - S. 1035-1037.
ISSN 1476-4679
DOI: https://doi.org/10.1038/ncb1467

Stemmann, Olaf ; Boos, Dominik ; Gorr, Ingo H.:
Anaphase Topsy-Turvy : Cdk1 a securin, separase a CKI.
In: Cell Cycle. Bd. 5 (2006) Heft 1 . - S. 11-13.
ISSN 1551-4005
DOI: https://doi.org/10.4161/cc.5.1.2296

2005

Gorr, Ingo H. ; Boos, Dominik ; Stemmann, Olaf:
Mutual Inhibition of Separase and Cdk1 by Two-Step Complex Formation.
In: Molecular Cell. Bd. 19 (1 Juli 2005) Heft 1 . - S. 135-141.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2005.05.022

Viadiu, Hector ; Stemmann, Olaf ; Kirschner, Marc W. ; Walz, Thomas:
Domain structure of separase and its binding to securin as determined by EM.
In: Nature Structural & Molecular Biology. Bd. 12 (Juni 2005) . - S. 552-553.
ISSN 1545-9985
DOI: https://doi.org/10.1038/nsmb935

Stemmann, Olaf ; Boos, Dominik ; Gorr, Ingo H.:
Rephrasing anaphase : Separase FEARs shugoshin.
In: Chromosoma. Bd. 113 (2005) Heft 8 . - S. 409-417.
ISSN 1432-0886
DOI: https://doi.org/10.1007/s00412-005-0331-y

Pfleghaar, Katrin ; Heubes, Simone ; Cox, Jürgen ; Stemmann, Olaf ; Speicher, Michael R.:
Securin Is Not Required for Chromosomal Stability in Human Cells.
In: PLoS Biology. Bd. 3 (2005) Heft 12 . - No. 0030416.
ISSN 1545-7885
DOI: https://doi.org/10.1371/journal.pbio.0030416

2003

Gerber, Scott A. ; Rusch, John ; Stemmann, Olaf ; Kirschner, Marc W. ; Gygi, Steven P.:
Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS.
In: Proceedings of the National Academy of Sciences of the United States of America. Bd. 100 (2003) Heft 12 . - S. 6940-6945.
ISSN 1091-6490
DOI: https://doi.org/10.1073/pnas.0832254100

2002

Zou, Hui ; Stemmann, Olaf ; Anderson, Jens S. ; Mann, Matthias ; Kirschner, Marc W.:
Anaphase specific auto-cleavage of separase.
In: FEBS Letters. Bd. 528 (2002) Heft 1-3 . - S. 246-250.
ISSN 1873-3468
DOI: https://doi.org/10.1016/S0014-5793(02)03238-6

Stemmann, Olaf ; Neidig, Anke ; Köcher, Thomas ; Wilm, Matthias ; Lechner, Johannes:
Hsp90 enables Ctf13p/Skp1p to nucleate the budding yeast kinetochore.
In: Proceedings of the National Academy of Sciences of the United States of America. Bd. 99 (2002) Heft 13 . - S. 8585-8590.
ISSN 1091-6490
DOI: https://doi.org/10.1073/pnas.082223899

2001

Stemmann, Olaf ; Zou, Hui ; Gerber, Scott A. ; Gygi, Steven P. ; Kirschner, Marc W.:
Dual inhibition of sister chromatid separation at metaphase.
In: Cell. Bd. 107 (2001) Heft 6 . - S. 715-726.
ISSN 1097-4172
DOI: https://doi.org/10.1016/S0092-8674(01)00603-1

1999

Pietrasanta, Lía I. ; Thrower, Douglas ; Hsieh, Wan ; Rao, Shashirekha ; Stemmann, Olaf ; Lechner, Johannes ; Carbon, John ; Hansma, Helen:
Probing the Saccharomyces cerevisiae centromeric DNA (CEN DNA)-binding factor 3 (CBF3) kinetochore complex by using atomic force microscopy.
In: Proceedings of the National Academy of Sciences of the United States of America. Bd. 96 (1999) Heft 7 . - S. 3757-3762.
ISSN 1091-6490
DOI: https://doi.org/10.1073/pnas.96.7.3757

Ortiz, Jennifer ; Stemmann, Olaf ; Rank, Simone ; Lechner, Johannes:
A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore.
In: Genes & Development. Bd. 13 (1999) . - S. 1140-1155.
ISSN 1549-5477

1996

Brikun, I. ; Suziedelis, K. ; Stemmann, Olaf ; Zhong, R. ; Alikhanian, L. ; Linkova, E. ; Mironov, A. ; Berg, D. E.:
Analysis of CRP-CytR interactions at the Escherichia coli udp promoter.
In: Journal of Bacteriology. Bd. 178 (1996) Heft 6 . - S. 1614-1622.
ISSN 1098-5530

Stemmann, Olaf ; Lechner, Johannes:
The Saccharomyces cerevisiae kinetochore contains a cyclin-CDK complexing homologue, as identified by in vitro reconstitution.
In: The EMBO Journal. Bd. 15 (1996) Heft 14 . - S. 3611-3620.
ISSN 1460-2075